FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21194
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC795237.52005284514485No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT459421.675946022459186No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA249011.748608096631122No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA17968.474096442389357No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG4802.264791922242144No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2741.2928187222798906No Hit
TGCCATCTTGCCACATCTCCTCTTAAACACCATTAATCACCTCTCTAGGG1170.5520430310465226No Hit
TGCCATCTTGCCACATCTTCTCGGGCATCTCTCTCTCTGAAGATCTCCGC990.46711333396244226No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT880.41521185241105973No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGGACATGGTC760.35859205435500613No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC660.3114088893082948No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACCAGTCAAGACCAAGTCT630.2972539397942814No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG610.28781730678493916No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT540.2547890912522412No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT480.22647919222421442No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT480.22647919222421442No Hit
TGCCATCTTGCCACATCTTCTCTTAAACACCATTAATCACCTCTCTAGGG460.21704255921487212No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT400.1887326601868453No Hit
TGCCATCTTCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT390.1840143436821742No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC380.17929602717750306No Hit
TGCCTCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT370.17457771067283193No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT320.15098612814947626No Hit
TGCCATCTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT320.15098612814947626No Hit
TCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT320.15098612814947626No Hit
GAGGACATCTTATGGTGCACTGCAGCCTCAAACTCCTGGCCTCAAGCAGC310.14626781164480512No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT270.1273945456261206No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACCAGTC270.1273945456261206No Hit
TGCCATCTTGCCACATCTTGGAAGTTTAGTTGAAATCATTGAACATGGTC250.11795791261677833No Hit
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT240.11323959611210721No Hit
TGCCATCTTGCCACTCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT240.11323959611210721No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC240.11323959611210721No Hit
TGCCATCTTGCCACATCTGCTCCAAGCAGTGGTATCAACGCAGAGTACAT230.10852127960743606Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)