FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21194
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length138-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG1108552.302538454279514No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC236011.135226951023874No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC17538.271208832688497No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC4742.236482023214117No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2971.4013400018873265No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC2681.2645088232518638No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2561.20788902519581No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2451.1559875436444278No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2050.9672548834575823No Hit
GTTGGCGGATACCCTTCAAATGGTCATGTGATGAGTCAGTGTTTCTATTA1370.6464093611399453No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1310.6180994621119185No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1180.5567613475511937No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1110.5237331320184958No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1030.48598659998112675No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA910.42936680192507315No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA860.4057752194017174No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG650.30669057280362366No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCATTGCCATGTAGAACCAT630.2972539397942814No Hit
GTTGGCGGATACCCTTCAGAGAGAGGACGCCCGAGAAGACAGGCGGTGGC620.29253562328961025No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT400.1887326601868453No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG380.17929602717750306No Hit
GTTGGCGGATACTCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG340.16042276115881854No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCCATAAAGGCTTTA320.15098612814947626No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT320.15098612814947626No Hit
GTTGGCGAATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG310.14626781164480512No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCATTGCC280.13211286213079174No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAGGATTCG260.12267622912144947No Hit
GTTGGCGGATACCCTTCCGAGAGAGGACGCCCGAGAAGACAGGCGGTGGC260.12267622912144947No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA250.11795791261677833No Hit
GTTGGCGGATACCCTTCCATAAAGGCCTTAACACAGAATCAAAAGATTCG240.11323959611210721No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGGTTCG230.10852127960743606No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)