FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15234
Total Bases2.2 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA694245.56912170145726No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACCAGTCA12077.923066824209006No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT9526.249179466981751No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAA6454.233950374163056Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG5043.308389129578574No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2851.870815281606932No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAAACGCAGAGTACATGGGA2481.62793750820533No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTA2011.319417093343836Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACCAGTC1871.227517395299987No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGAA910.5973480372850204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAG690.45293422607325723Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTCCGGAAGCAGTGGTATCAACGCAGAGTACAT680.44636996192726797Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGGT620.4069843770513325Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA620.4069843770513325No Hit
TGCCATCTTGCCACATCTTAGGTAGTATCAACGCAGAGTACATGGGAAGC610.40042011290534335No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAC590.3872915846133649Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTACGTCCTTGGGCGAAAAGGGTTGGACATCATC520.3413417355914402No Hit
TGCCATCTTGCCACATCTGCCCGGAAGCAGTGGTATCAACGCAGAGTACA510.334777471445451Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGTA490.3216489431534725Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACCAGTCAA490.3216489431534725No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGGA470.30852041486149406Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC460.3019561507155048No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGAAG410.2691348299855586Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAT400.2625705658395694Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACCA370.2428777734016017No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACAGTCAA340.22318498096363398No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGGG320.2100564526716555Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTGGGTAGTATCAACGCAGAGTACATGGGAAGC310.20349218852566625No Hit
TGCCATCTTGCCACATCTGGGGTAGTATCAACGCAGAGTACATGGGAAGC300.19692792437967704No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTG250.16410660364973087Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGAAA240.15754233950374164Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC240.15754233950374164No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACCAGTCAA240.15754233950374164No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCCCAACCAGTCAA230.1509780753577524No Hit
GAGGACATCTTATGGTGCACTGAAAAGGGTGGGGATGGGAGGTTTAGGGC220.14441381121176317No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT220.14441381121176317No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACACAGC220.14441381121176317No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTACCGCCAACCAGTCAA220.14441381121176317No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT210.13784954706577393No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCACCAGTCAA210.13784954706577393No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACCAGTC210.13784954706577393No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAAACGCAGAGTACATGGGA200.1312852829197847No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGCC200.1312852829197847No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA200.1312852829197847Clontech Universal Primer Mix Long (96% over 25bp)
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGAG190.12472101877379546Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACCA170.11159249048181699No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATCTGGTTTCCCAGTCCACTA170.11159249048181699No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA170.11159249048181699No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC170.11159249048181699No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG160.10502822633582776No Hit
TGCCCTCTCTGCCTTTTCTCCCCAGGGTATTTGGTTTCCCAGTCCACTAT160.10502822633582776No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCGCTA160.10502822633582776No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGATCCGCCAACCAGTCAA160.10502822633582776No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)