FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19812
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC656933.15667272360186No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG497725.12113870381587No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC282714.269129820310923No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC5062.5540076721179084No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC3031.5293761356753481No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1400.7066424389259035No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA980.4946497072481324No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT970.4896022612558046No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA930.469412477286493No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA900.4542701393095093No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG690.3482737734706239No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG640.3230365435089845No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT610.3078942055320008No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT610.3078942055320008No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA600.30284675953967294No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA560.2826569755703614No Hit
GTTGGCGGATACCCTTCCGAGACGCGACCTCAGATCAGATGTGGCAAGAT540.27256208358570566No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA480.24227740763173833No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA460.2321825156470826No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCATT440.2220876236624268No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA430.21704017767009892No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT370.18675550171613164No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA370.18675550171613164No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC330.1665657177468201Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTGATGTGGCAAGATGGCATTGCCATGTAGAACCAT320.16151827175449224No Hit
TACCTCAGTTTGCCCCCATGGGAAGCAGTGGTATCAACGCAGAGTACATG320.16151827175449224Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC260.13123359580052493No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCTTGCCATGTAGAACCATGTC250.12618614980819706No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT240.12113870381586916No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC240.12113870381586916No Hit
GTTGGCGGATACCCTTGGACTTTCGGCGAGAGTGTCTTTCACACTCTTTG220.1110438118312134No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCATT220.1110438118312134No Hit
GTTGGCGGATACCCTTGACGATGAAGTAGATGTGGCAAGATGGCATTGCC220.1110438118312134No Hit
GTTGGCGGATACCCTTCCGAGACGCGACCTCAGATAAGATGTGGCAAGAT210.10599636583888553No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT200.10094891984655764No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT200.10094891984655764No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT200.10094891984655764No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)