FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13697
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA849362.00627874717092No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA184313.455501204643353No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG5413.9497700226326935No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG3412.489596261955173No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT2551.8617215448638387No Hit
TGCCATCTTGCCACATCTCTCAGGACCGACTGACCCATGTTCAACTGCTG1020.7446886179455355No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC670.4891582098269694No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC480.350441702562605No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT430.3139373585456669No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGCAAGGGT400.2920347521355041No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT400.2920347521355041No Hit
TGCCATCTTGCCACATCTTAGGACGGTGGCCATGGAAGGGTATCCGCCAA320.23362780170840333No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC320.23362780170840333No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT310.2263269329050157No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT280.20442432649485287No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA260.18982258888807768No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACCA260.18982258888807768No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT250.1825217200846901No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCGCTA220.16061911367452728No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACCA210.15331824487113968No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTACTTGGTTTCCCAGTCCACTA180.13141563846097687No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA180.13141563846097687No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGCCCACTA170.12411476965758926No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGTCAAGGGTATCCGCCAAC160.11681390085420167No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT160.11681390085420167No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACCAGTC160.11681390085420167No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCGGTCCACTA160.11681390085420167No Hit
TGCCCTCTCTGCCTTTTCCCCCCCAGGGTATTTGGTTTCCCAGTCCACTA160.11681390085420167No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG150.10951303205081406Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTA150.10951303205081406Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT150.10951303205081406No Hit
TGCCATCTTGCCACATCTGTGCCAAGGGTATCCGCCAACCAGTCAAGACC150.10951303205081406No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTCGGTTTCCCAGTCCACTA150.10951303205081406No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG150.10951303205081406No Hit
TGCCATCTTGCCACATCTTTCAGGACCGACTGACCCATGTTCAACTGCTG140.10221216324742644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)