FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13697
Total Bases1.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC810659.180842520259915No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC176712.900635175585895No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC5263.8402569905818793No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC3342.43849018033146No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG2541.8544206760604511No Hit
TACCTCAGTTTGCCCCCGGAGCCCCGCGGACGCTACGCCGCGACGAGTAG880.6424764546981091No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG620.4526538658100314No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT520.37964517777615536No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG470.34314083375921733No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT410.2993356209388917No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT320.23362780170840333No Hit
GTTGGCGGATACCCTTGCTGAGCCAGCATTAGCAGCTGGTACAGATGTGG320.23362780170840333No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT300.21902606410162812No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA290.2117251952982405No Hit
GTTGGCGGATACCCTTCCATGGCCACCGTCCTAAGATGTGGCAAGATGGC280.20442432649485287No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATAC260.18982258888807768No Hit
GTGTCGCTACCTCAGTTTGCACCCATGTCCCTTACACACACGCAAAATAC230.16791998247791487No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCATT230.16791998247791487No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC230.16791998247791487No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATGC220.16061911367452728No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT210.15331824487113968No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC210.15331824487113968No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT190.13871650726436446No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTACCTTACACACACGCAAAATAC180.13141563846097687No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC180.13141563846097687No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC170.12411476965758926No Hit
GTTGGCGGATACCCTTGGCACAGATGTGGCAAGATGGCATTGCCATGTAG150.10951303205081406No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC150.10951303205081406Clontech Universal Primer Mix Long (96% over 26bp)
TACCTCAGTTTGCCCCCATGAGATGTCCTCTTGCCATGTAGAACCATGTC150.10951303205081406No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT150.10951303205081406No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC150.10951303205081406No Hit
GTGTCGCTACCTCAGTTTGCCTCCATGTCCCTTACACACACGCAAAATAC150.10951303205081406No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATAC140.10221216324742644No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCATTGCC140.10221216324742644No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT140.10221216324742644No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACATGCAAAATAC140.10221216324742644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)