FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26098
Total Bases3.7 Mbp
Sequences flagged as poor quality0
Sequence length139-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA632624.239405318415205No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC566521.706644187294046No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA483718.533987278718676No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT15515.942984136715457No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACCAGTCA8823.379569315656372No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTA5582.138094873170358Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAA5382.0614606483255424Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGTA2801.0728791478274198Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2400.9196106981377883No Hit
TGCCATCTTGCCACATCTGTCAGACAAGGCCTTCTCCTGACAGTGGGGGC2030.7778373821748793No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1790.6858763123611005No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGAA1440.551766418882673Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGGT1240.4751321940378573Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1130.43298337037320866No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACCAGTC1090.4176565254042456No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT810.31036861062150356No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT620.23756609701892864No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT620.23756609701892864No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTG580.22223925204996553Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAT550.21074411832324316Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAG550.21074411832324316Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGGT480.18392213962755766Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC410.15710016093187218No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT410.15710016093187218No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT370.14177331596290904No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC360.13794160472066824No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA340.13027818223618667No Hit
TGCCATCTTGCCACATCTTAGCAACCCCACACTGCTTAAAGAAGTCTGCC330.12644647099394588No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGGA320.12261475975170512Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT310.11878304850946432No Hit
TGCCTCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT280.10728791478274197No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT270.1034562035405012No Hit
TGCCATCTTGCCACATCCAAGCAGTGGTATCAACGCAGAGTACATGGGTA270.1034562035405012Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)