FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26098
Total Bases3.7 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG630424.155107671085908No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC614723.553529006054102No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC472918.12016246455667No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCA8633.3067668020537977No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT4001.5326844968963138No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC2370.9081155644110659No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1720.659054333665415No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1710.6552226224231742No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC1620.6207372212430071Clontech Universal Primer Mix Long (96% over 26bp)
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1450.5555981301249138No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1430.5479347076404322No Hit
GTTGGCGGATACCCTTCCAAATCATCACATCCAAAACTCAGTTCACAAGC1420.5441029963981914No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTAAGATGTGGC1370.5249444401869875Clontech SMART CDS Primer II A (96% over 25bp)
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCTTGCC1090.4176565254042456No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA980.3755077017395969No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTAGATGTGGC900.34485401180167063Clontech SMART CDS Primer II A (96% over 26bp)
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT720.2758832094413365No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA710.2720514981990957No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA690.26438807571461415No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA680.26055636447237335No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA620.23756609701892864No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA490.18775385086979846No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT450.17242700590083532No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA450.17242700590083532No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGATGGCGGAT440.16859529465859455No Hit
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT430.16476358341635375No Hit
GTTGGCGGATACCCTTGGATGAAGGACCAGATCTTGATCTAGGCCCACCT400.15326844968963138No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG400.15326844968963138No Hit
GTTGGCGGATACCCTTCCATATCATCACATCCAAAACTCAGTTCACAAGC380.14560502720514984No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT370.14177331596290904No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG370.14177331596290904No Hit
GTTGGCGGATACCCTTGTACTCTGCGTTGATACCACCCCATGTACTCTGC350.13410989347842747Clontech SMART CDS Primer II A (95% over 21bp)
CAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT310.11878304850946432No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)