FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18381
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC963552.41825798378761No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG322417.53985093302867No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC10405.658016430009249No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCA10005.440400413470432No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC2571.398182906261901No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1750.9520700723573255No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCTTGCC1650.8976660682226212No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA750.4080300310102824No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA740.4025896305968119No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA700.3808280289429302No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT590.3209836243947555No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT460.25025841901963986No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA450.24481801860616942No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA390.21217561612534683No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA370.20129481529840595No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATAC350.1904140144714651No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA340.18497361405799467No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA340.18497361405799467No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT280.15233121157717208No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA280.15233121157717208No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC270.14689081116370165No Hit
GTTGGCGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT260.1414504107502312No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC260.1414504107502312No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC240.13056960992329036No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT220.11968880909634949No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGAGGAT210.11424840868287905No Hit
TTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT210.11424840868287905No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC200.10880800826940863No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACAC200.10880800826940863No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA190.1033676078559382No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT190.1033676078559382No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)