FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15046
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1009767.10753688688024No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT197313.113119766050776No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG4653.0905223979795298No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2601.7280340289778016No Hit
TGCCATCTTGCCACATCTCTGATGGCGCGAGGGAAGGGTATCCGCCAACC1691.1232221188355709No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC660.4386547919712881No Hit
TGCCATCTTGCCACATCTCTGATGGCGCGAGGAAGGGTATCCGCCAACCA520.34560680579556025No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA500.3323142363418849No Hit
TGCCATCTTGCCACATCTCCTCTCTTCACTACTTCCCTACATTAAAGCAG490.3256679516150472No Hit
TGCCATCTTGCCACATCTTTGATGGCGCGAGGGAAGGGTATCCGCCAACC480.3190216668882095No Hit
TGCCATCTTGCCACATCTTAGATGGCGCGAGGGAAGGGTATCCGCCAACC430.285790243254021No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC420.27914395852718327No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT320.21268111125880632No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT320.21268111125880632No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA250.16615711817094245No Hit
TGCCATCTTGCCACATCTCTGATGGAAGGGTATCCGCCAACCAGTCAAGA230.15286454871726704No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC230.15286454871726704No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT220.14621826399042934No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG220.14621826399042934Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTCTCAGGACCGACTGACCCATGTTCAACTGCTG220.14621826399042934No Hit
TGCCATCTTGCCACATCTTCTCTTGGAATCAACATCTTCAAGCAGTGGTA210.13957197926359163No Hit
TGCCATCTTGCCACATCTTCTTCCTCTTCCTCGAAGGGTATCCGCCAACC210.13957197926359163No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA210.13957197926359163No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTCGGTTTCCCAGTCCACTA190.12627940980991625No Hit
TGCCCTCTCTGCCTTTTCTCCCCAGGGTATTTGGTTTCCCAGTCCACTAT180.11963312508307855No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGCATTTGGTTTCCCAGTCCACTA170.11298684035624086No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGCCCACTA170.11298684035624086No Hit
TGCCATCTTGCCACATCTTCTCTCTTCACTACTTCCCTACATTAAAGCAG160.10634055562940316No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCGCTA160.10634055562940316No Hit
TGCCCTCTCTGCCTTTTCCCCCCCAGGGTATTTGGTTTCCCAGTCCACTA160.10634055562940316No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)