FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008153999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008153999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20119
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1043951.886276653909235No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA257512.798846861176003No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC16858.375167751876337No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT13236.575873552363437No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG9254.597644018092351No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG5282.6243849097867686No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC1210.6014215418261345No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC800.39763407724041955No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT570.2833142800337989No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT380.18887618668919928No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA340.16899448282717827No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT330.16402405686167304No Hit
GAGGACATCTTATGGTGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCT330.16402405686167304No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT310.15408320493066258No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC310.15408320493066258No Hit
TGCCATCTTGCCACATCTACGTCCTTGGACGAAGGGTATCCGCCAACCAG300.14911277896515732No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT290.14414235299965206No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG270.13420150106864157No Hit
TGCCATCTTGCCACATCTCTCCTTGGATGGCAGGGGCAAATGGGAGCCTG250.1242606491376311No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCGCTA250.1242606491376311No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACCAGTC250.1242606491376311No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA250.1242606491376311No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT210.1043789452756101No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT210.1043789452756101No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)