FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9397
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA276829.456209428541026No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA191920.42141108864531No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT8258.779397680110673No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACCAGTCA6396.80004256677663No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC5575.927423645844419No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2482.6391401511120574No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1831.9474300308609132No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACCAGTC790.8406938384590827No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT710.7555602851974035No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA710.7555602851974035Clontech Universal Primer Mix Long (96% over 25bp)
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA630.6704267319357242No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT360.3831009896775567No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC340.36181760136213686No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT340.36181760136213686No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT310.32989251888900717No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT290.3086091305735873No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT270.28732574225816754No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG260.2766840481004576Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTACCTCATTAGCATCCACTGACACATTAGCCTG260.2766840481004576No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT210.22347557731190806No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACCAGTC210.22347557731190806No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC200.21283388315419816No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC200.21283388315419816No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACCAGTCAA170.18090880068106843No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACAGTCAA150.15962541236564862No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT140.1489837182079387No Hit
AAGGGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT140.1489837182079387No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACCAGTCAAGACCAAGTCT140.1489837182079387No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACCAGTCAA130.1383420240502288No Hit
TGCCATCTTGCCACATCTATACCAGCTGCTAATGCTGGCTCAGCAAGGGT130.1383420240502288No Hit
TGCCATCTTGCCACATCTTATGTCTAGTTATAGGCCAATGAATGTTATTC120.1277003298925189No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACCA110.11705863573480899No Hit
AAGGTGGATGGTGATGTGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT100.10641694157709908No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACCA100.10641694157709908No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)