FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41768
Total Bases6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1717641.12239034667688No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA1073125.69191725723042No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT581213.914958820149398No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC26976.457096341696992No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG4951.1851177935261443No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG3640.8714805592798315No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA2550.6105152269680137No Hit
AAGGTGGATGGTGATGTGGTCCCCGATGACCCTGAGATCCTCATGCAGCA1640.3926450871480559No Hit
TGCCATCTTGCCACATCTTTTCTGCGGGATGAACCGAACGCCGGGTTAAG1260.30166634744301857No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC620.14843899636085042No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT590.14125646427887378No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT540.1292855774755794No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT460.11013215859030838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)