FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38953
Total Bases5.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1556939.968680204348836No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA840121.567016661104407No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT759319.49272199830565No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC23506.032911457397376No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG5501.411958000667471No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG3690.9472954586296305No Hit
TGCCATCTTGCCACATCTTATTCAAAAGATTTTGGTGGAGGCACTACCAA1950.5006032911457398No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC890.2284804764716453No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT850.21821169101224552No Hit
TGCCATCTTGCCACATCTTAGCATTGGGCATTGATGTTCAGAATGACTTT540.13862860370189717No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC510.13092701460734732No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT450.11552383641824764No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT400.10268785459399789No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT390.10012065822914795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)