FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17681
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length91-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG670937.9446863865166No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC498828.21107403427408No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC5373.0371585317572536No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC3261.8437871161133421No Hit
GTTGGCGGATACCCTTCAAGATGTGGCAAGATGGCATTGCCATGTAGAAC2341.3234545557377975No Hit
GTTGGCGGATACCCTTCCAGATGTGGCAAGATGGCATTGCCATGTAGAAC2111.1933714156439115No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2091.1820598382444432No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1580.8936146145580002No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1350.7635314744641141No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1270.7182851648662405No Hit
GTTGGCGGATACCCTTCCTCTTCAGATGTGGCAAGATGGCATTGCCATGT1010.5712346586731519No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT980.5542672925739494No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA850.48074203947740507No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA710.40156099768112663No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA640.3619704767829874No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA590.3336915332843165No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG580.3280357445845823No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA580.3280357445845823No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG550.31106837848537977No Hit
GTTGGCGGATACCCTTCTGCCTCCCGGCTGGACAGAGTGTGGACTTCCCC490.2771336462869747No Hit
GTTGGCGGATACCCTTCGCCGCCCGAAGATCTAAGATGTGGCAAGATGGC490.2771336462869747No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT480.2714778575872405No Hit
GTTGGCGGATACCCTTCCCGGCGGATAGAGATGTGGCAAGATGGCATTGC450.254510491488038No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA450.254510491488038No Hit
GTTGGCGGATACCCTTCCATAAGGCTTTAACACAGAATCAAAAGATTCGA380.21491997058989878No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC330.18664102709122787Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCATT300.16967366099202533No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCATTGCC280.158362083592557No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC280.158362083592557No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCCATAAAGGCTTTA250.14139471749335444No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAAAATCAAAAGATTCG240.13573892879362026No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCATTGCCATGTAGAACCAT200.11311577399468356No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC200.11311577399468356No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC190.10745998529494939No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCTTGCCATGTAGAACCATGTC190.10745998529494939No Hit
GTTGGCGGATACCCTTCCTCCCCACTCCACCCCAAGTACAGTTCAGAGGC180.1018041965952152No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC180.1018041965952152No Hit
GTTGGCGGATACTCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG180.1018041965952152No Hit
GTTGGCGGATACCCTTAGCCGCCCGAAGATCTAAGATGTGGCAAGATGGC180.1018041965952152No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)