FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16587
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA732044.1309459215048No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC243314.668113582926386No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT176710.652920962199312No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA13117.903780068728522No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACCAGTCA9465.703261590402122No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACCAGTC1801.0851871947911014No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1450.8741785735817206No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1050.6330258636281425No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA1010.6089105926327847No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT420.253210345451257No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT310.18689335021402304No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACCAGTCAA310.18689335021402304No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.16880689696750467No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC260.15674926146982576No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC250.1507204437209863No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG240.14469162597214685No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACAGTCAA220.13263399047446794No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT220.13263399047446794No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCAACCAGTCAA220.13263399047446794No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACCA190.11454753722794961No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTACCGCCAACCAGTCAA180.10851871947911015No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT170.10248990173027069No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)