FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9505
Total Bases1.3 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT736877.51709626512363No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG6316.638611257233035No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG4154.36612309310889No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC770.8100999473961072No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC500.5260389268805892No Hit
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGG270.2840610205155181Clontech Universal Primer Mix Long (96% over 27bp)
GAGGACATCTTATGGTGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCT260.2735402419779064No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT220.23145712782745922No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT210.22093634928984746No Hit
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG210.22093634928984746Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT170.17885323513940032No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG160.16833245660178853Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC140.14729089952656496No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT140.14729089952656496No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC130.1367701209889532No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG120.1262493424513414No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGAGTATTTGGTTTCCCAGT110.11572856391372961No Hit
GGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC110.11572856391372961No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTCGGTTTCCCAGT110.11572856391372961No Hit
GAGGACATCTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTC110.11572856391372961No Hit
GAGGACATCTTATGGTGCATTGAAATGGAAAGGGTATTTGGTTTCCCAGT100.10520778537611783No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACCAGTCAAGACCAAGTCT100.10520778537611783No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTGTTTGGTTTCCCAGT100.10520778537611783No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTCCCCAGT100.10520778537611783No Hit

[FAIL]Adapter Content

Adapter graph