FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27066
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC996736.8247986403606No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA602522.26040050247543No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC397914.701101012340207No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG24479.040863075445207No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCA3671.3559447277026526No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1130.4174979679302446No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC960.35468853912657944No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT870.32143648858346263No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG720.2660164043449346No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT550.20320697554126946No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCTTGCC540.19951230325870095No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA530.19581763097613242No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA520.19212295869356388No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT470.17364959728072119No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG470.17364959728072119No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG460.16995492499815268No Hit
GTTGGCGGATACCCTTCCACACACGCAAAATACTCCTTCAGCGGAGCGAA430.15887090815044705No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC410.15148156358530998No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT390.1440922190201729No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT380.14039754673760438No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA370.13670287445503584No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG360.1330082021724673No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC340.12561885760733021No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA340.12561885760733021No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA340.12561885760733021No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA340.12561885760733021No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC330.1219241853247617No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG320.11822951304219316No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT320.11822951304219316No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC290.10714549619448754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)