FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27526
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1138941.375426869141904No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA406314.760589987648043No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA301910.96781225023614No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT27369.939693380803604No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC14435.242316355445761No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG6352.3069098307055147No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACCAGTCA4571.66024849233452No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG4081.482234977839134No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC950.345128242389014No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGTCACCTGCTTCCGGGCTGA940.3414953135217612No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT920.33422945578725566No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACCAGTC900.3269635980527501No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT630.22887451863692507No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT590.21434280316791396No Hit
TGCCATCTTGCCACATCTCTGATGGCGCGAGGGAAGGGTATCCGCCAACC480.1743805856281334No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT480.1743805856281334No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC420.15258301242461672No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACCAGTC400.14531715469011117No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT370.13441836808835284No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT350.12715251035384725No Hit
TGCCATCTTGCCACATCTTCCTTAGCATTCTGTTCTTTGAAATGGAAGGG330.11988665261934173No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGGTATTTGGTTTCCCAGT300.10898786601758338No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT290.10535493715033059No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC290.10535493715033059No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)