FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32916
Total Bases4.7 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1250037.97545266739579No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA567017.225665329930735No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA462214.041803378296269No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC338710.289828654757564No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT21326.477093206951026No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG4181.2698991371977153No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2520.7655851257746992No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT1140.3463361283266497No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG1080.32810791104629966No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT650.1974723538704581No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT650.1974723538704581No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT570.17316806416332484No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC490.14886377445619153No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT450.13671162960262487No Hit
TGCCATCTTGCCACATCTTGTTCAGTGGAGATCCAAGATTGGGGCTGAGG440.1336735933892332No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT420.12759752096244986No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC380.11544537610888321No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT360.10936930368209989No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC350.10633126746870823No Hit
TGCCATCTTGCCACATCTTTTCCCTGTCAGGATCAGCTTGGAACCAAAAG340.10329323125531657No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT330.10025519504192489No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT330.10025519504192489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)