FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18669
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC699537.468530719374364No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG598732.069205635009915No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC5743.07461567304087No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC3391.8158444480154265No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1630.8731051475708393No Hit
TACCTCAGTTTGCCCCCGGAGCCCCCAGCAGTGCCCTCTGAGGTGGAGCT1540.8248968878890138No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1320.7070544753334405No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1190.6374203224596925No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1150.6159944292677701No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1140.6106379559697895No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA780.41780491724248753No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA730.3910225507525845No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA710.3803096041566233No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG710.3803096041566233No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCATTGCC550.29460603138893354No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA540.28924955809095293No Hit
TACCTCAGTTTGCCCCCAGCAGTGCCCTCTGAGGTGGAGCTGCAGCACGT510.2731801381970111No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA460.24639777170710805No Hit
GTTGGCGGATACCCTTGTGCTGAGCCAGCATAGCAGCTGGTCAGATGTGG410.21961540521720496No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG400.21425893191922438No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCATT340.18212009213134073No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCAGTGCACC330.17676361883336011Clontech SMART CDS Primer II A (96% over 26bp)
GTTGGCGGATACCCTTGCCCCCGGAGCCCCCAGCAGTGCCCTCTGAGGTG310.1660506722373989No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT310.1660506722373989No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC280.14998125234345708No Hit
GTTGGCGGATACCCTTCCATCTTTGTCAGAAAACATTAATGTTGCCATCC270.14462477904547646No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCATTGCCATGTAGAACCAT260.13926830574749585No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT260.13926830574749585No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC240.12855535915153463Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTCGAATGGCGTGCCTCTCATGGTTCTGGATGGGAG240.12855535915153463No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC230.12319888585355403No Hit
GTTGGCGGATACCCTTCCCTCGCGCCAGGTAGATGTGGCAAGATGGCATT220.11784241255557341No Hit
GTTGGCGGATACCCTTCCCCCGGAGCCCCCAGCAGTGCCCTCTGAGGTGG210.11248593925759282No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTAC200.10712946595961219No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC190.10177299266163158No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA190.10177299266163158No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)