FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24211
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1057443.67436289289992No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA817333.75738300772376No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA6142.536037338399901No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG5182.139523357151708No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT3521.4538845979100408No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG3521.4538845979100408No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC1610.6649869893849903No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC580.2395605303374499No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC570.23543017636611457No Hit
TGCCATCTTGCCACATCTGTACCAGCTGTATGCTGGCTCAGCAAGGGTAT560.23129982239477923No Hit
AAGGTGGATGGTGATGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT480.19825699062409646No Hit
TGCCATCTTGCCACATCTGCAGTGGTATCAACGCAGAGTACATGGGAAGC410.16934451282474908Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT350.144562388996737No Hit
TGCCATCTTGCCACATCAGCAGTGGTATCAACGCAGAGTACATGGGAAGC330.13630168105406634Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT280.11564991119738961No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGC270.11151955722605428No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC260.10738920325471893No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)