FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22791
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA841136.904918608222545No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC423318.573120968803476No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCA22179.727524022640516No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG21979.63977008468255No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC3761.6497740336097584No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCTTGCC3001.3163090693694879No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC2140.9389671361502347No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT780.3422403580360669No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG780.3422403580360669No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT730.3203018735465754No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG690.30275108595498224No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG640.28081260146549075No Hit
GTTGGCGGATACCCTTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCC570.2500987231802027No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA570.2500987231802027No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA550.24132332938440615No Hit
GTTGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT540.23693563248650784No Hit
GTTGGCGGATACCCTTCCTTGTAGTAAGACACCAACTGCTTAAAACATAA530.23254793558860953No Hit
GTTGGCGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT520.22816023869071125No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT480.21060945109911805No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG470.2062217542012198No Hit
TTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT470.2062217542012198No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA460.2018340573033215No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA460.2018340573033215No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG450.1974463604054232No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT440.1930586635075249No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG390.17112017901803345No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC380.16673248212013514No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA370.16234478522223686No Hit
GTTGGCGGTACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT360.15795708832433855No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT350.15356939142644024No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT320.14040630073274538No Hit
GTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT290.12724321003905048No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG290.12724321003905048No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG280.12285551314115221No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA280.12285551314115221No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT270.11846781624325392No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT250.10969242244745732No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA250.10969242244745732No Hit
GTTGGCGGATCCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT250.10969242244745732No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA250.10969242244745732No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT250.10969242244745732No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA240.10530472554955903No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA240.10530472554955903No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG240.10530472554955903No Hit
GTTGGCGGATACCCTTCCATGTAGTAAGACACCAACTGCTTAAAACATAA230.10091702865166075No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG230.10091702865166075No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG230.10091702865166075No Hit
CGAAGTAGACCAGCGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT230.10091702865166075No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)