FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22103
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC568325.711441885716873No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA495522.41777134325657No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC426419.291498891553182No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG14286.460661448672125No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC4381.9816314527439716No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1840.8324661810613945No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT1120.5067185449938922No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1010.456951545039135No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC970.43885445414649593Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTCGACATGAAGCCCTTTCTGATCACTGTCCTAGAA540.24431072705062662No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC540.24431072705062662No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT520.23526218160430712No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG490.22168936343482784No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG490.22168936343482784No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT480.21716509071166812No Hit
GTTGGCGGATACCCTTCCATTTCAAAGAACAGAATGCTAAGGAAGATGTG430.19454372709586934No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA400.1809709089263901No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT390.17644663620323034No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA370.1673980907569108No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG360.1628738180337511No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT360.1628738180337511No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG350.1583495453105913No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT350.1583495453105913No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG350.1583495453105913No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA350.1583495453105913No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG340.15382527258743156No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT340.15382527258743156No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA320.1447767271411121No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTAAGATGTGGC320.1447767271411121Clontech SMART CDS Primer II A (96% over 25bp)
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT320.1447767271411121No Hit
GTTGGCGGATACCCTTGCTCTGTGTTGGCGGATACCCATGTACTCTGCGT310.1402524544179523No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG310.1402524544179523No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA300.13572818169479256No Hit
CGAAGAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT290.1312039089716328No Hit
CAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT280.12667963624847306No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG270.12215536352531331No Hit
GTTGGCGGATACCCTTCCTTCTTTTTGAGATGTGGCAAGATGGCATTGCC250.1131068180789938No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA240.10858254535583406No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGGCCTTAGCTCAGCAC230.10405827263267431No Hit
GTTGGCGGATACCCTTCCGATCACACCACAGACAGCTACCGTGGCCTTTG230.10405827263267431No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)