FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15948
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC56

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC639840.117883120140455No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG253415.889139704038124No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA197612.39026837220968No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC13178.25808878856283No Hit
GTTGGCGGATACCCTTCCATTCACCAAAGATGATTGCTTTAATAGTTGTT3712.326310509154753No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1290.8088788562829194No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT1150.7210935540506647No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC890.5580637070479056No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT730.45773764735390016No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA690.4326561324303988No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT640.401304238776022No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC600.3762227238525207No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT530.3323300727363933No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA500.3135189365437672No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT460.28843742162026587No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA440.27589666415851516No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT410.2570855279658891No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT400.2508151492350138No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA400.2508151492350138No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT340.2131928768497617No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA330.20692249811888638No Hit
GAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT300.18811136192626035No Hit
CGAAGAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT300.18811136192626035No Hit
CAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT290.181840983195385No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT280.17557060446450964No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA260.16302984700275897No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG240.15048908954100826No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCCCCCTGGACCTTAGCTCAGCAC210.13167795334838223No Hit
CGAAGTAGACCAGCGGCACCTCGGCCCCACCCTGGACCTTAGCTCAGCAC210.13167795334838223No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCCC200.1254075746175069No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG190.11913719588663155No Hit
CGAAGTAGACCACGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT190.11913719588663155No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA190.11913719588663155No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG180.1128668171557562No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA170.10659643842488085No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCCGGACCTTAGCTCAGCAC170.10659643842488085No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCCCAGCAC160.1003260596940055No Hit
CGAAGTAGACCAGCGGCATCACCATCCACCTTTTGCCATGTAGAACCATG160.1003260596940055No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCGGCAC160.1003260596940055No Hit
CGAAGTAGACCAGCGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT160.1003260596940055No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA160.1003260596940055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)