FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22845
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA721331.573648500766033No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT468520.507769752681114No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT321914.090610636900855No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA257011.249726417159115No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC17477.647187568395711No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG3851.6852702998467937No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2511.0987086889910265No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC690.30203545633617856No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT640.28014882906544103No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT550.24075289997811336No Hit
TGCCATCTTGCCACATCTACGTCCTTGGGCGAAGGGTATCCGCCAACCAG540.23637557452396585No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC470.20573429634493326No Hit
TGCCATCTTGCCACATCTTTCCATCTGTCTCACATCCTTCTAAAACGAGG360.15758371634931057No Hit
TGCCATCTTGCCACATCTTAGGAAGAAAATGGACATCTAAATTCAGTAAG350.15320639089516305No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT340.14882906544101554No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT330.14445173998686803No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT300.13131976362442546No Hit
TGCCATCTTGCCACATCTTTCTTCTCCTCCCGCCTCCGACAGCGTTTCCA280.12256511271613044No Hit
TGCCATCTTGCCACATCTTTTCCCTGTCAGGATCAGCTTGGAACCAAAAG240.10505581089954039No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)