FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9712
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA423043.55436573311368No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT271427.944810543657333No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC116511.995469522240528No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1972.0284184514003294No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1151.1841021416803954No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG740.7619439868204283No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT300.3088962108731466No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA230.23682042833607905No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG230.23682042833607905No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT220.22652388797364084No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT180.18533772652388797No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC170.17504118616144976No Hit
TTTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT170.17504118616144976No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT160.16474464579901155No Hit
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT150.1544481054365733No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC150.1544481054365733No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA140.1441515650741351No Hit
TTCTGCGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT130.13385502471169688No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT130.13385502471169688No Hit
TGCCATCTTGCCACATCTTGACTCATTTACAGAAGTCAGCATTAATTAGT110.11326194398682042No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT110.11326194398682042No Hit
TTCTGCAGTTTGGCTGATAGTTGATATATTGAGAGAATCTGGATGATATT110.11326194398682042No Hit
TGCCCTCTCTGCCTTTTCTCCCCAGGGTATTTGGTTTCCCAGTCCACTAT100.10296540362438221No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACCAGTCAAGACCAAGTCT100.10296540362438221No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT100.10296540362438221No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGCCCACTA100.10296540362438221No Hit
TTCTGCAGTTTGGCTGAATAGTTGCTATATTGAGAGAATCTGGATGATAT100.10296540362438221No Hit
TTCTCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT100.10296540362438221No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)