FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20320
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA710334.955708661417326No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT364517.937992125984252No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT311815.344488188976376No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC256712.63287401574803No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA6663.27755905511811No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG5002.4606299212598426No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG3121.5354330708661417No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC1050.516732283464567No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC530.2608267716535433No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT440.21653543307086615No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC360.17716535433070865No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT340.1673228346456693No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG330.1624015748031496No Hit
TGCCATCTTGCCACATCTGACCCCATGATATGTATTGTTAACTGACAGAA310.15255905511811024No Hit
TGCCATCTTGCCACATCTGCTCCAAGCAGTGGTATCAACGCAGAGTACAT310.15255905511811024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT270.13287401574803148No Hit
TTCTGCGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT220.10826771653543307No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)