FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25177
Total Bases3.6 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1312952.14680065138817No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCA20158.00333637844064No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG19817.86829248917663No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC8173.2450252214322592No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC5082.0177145807681613No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCTTGCC3351.3305794971601064No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC1400.5560630734400445No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT1310.5203161615760417No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1300.5163442824800413No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG930.36938475592802955No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG930.36938475592802955No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG920.3654128768320292No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG790.3137784485840251No Hit
GTTGGCGGATACCCTTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCC710.28200341581602256No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG690.2740596576240219No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG680.2700877785280216No Hit
GTTGGCGGATACCCTTCCGGGCTCCTAAGATGTGGCAAGATGGCATTGCC550.21845335028001744No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG500.1985939548000159No Hit
GTTGGCGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT490.19462207570401557No Hit
GTTGGCGGATACCCTTGCTCCCTCGAGATGTGGCAAGATGGCATTGCCAT490.19462207570401557No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC470.18667831751201494Clontech Universal Primer Mix Long (96% over 26bp)
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA450.1787345593200143No Hit
TTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT450.1787345593200143No Hit
GTTGGCGGATACCCTTGGGCGAAGATGTGGCAAGATGGCATTGCCATGTA430.17079080112801368No Hit
GTTGGCGGTACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT430.17079080112801368No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA430.17079080112801368No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA390.15490328474401238No Hit
TACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATACTCCTTCA380.15093140564801208No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA360.14298764745601145No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA350.13901576836001112No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG350.13901576836001112No Hit
GTTGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT340.1350438892640108No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG330.13107201016801048No Hit
GTTGGCGGATACCCTTCCTAAGATGTGGCAAGATGGCATTGCCATGTAGA330.13107201016801048No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA330.13107201016801048No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCATGTACTCTGCGTTGA320.12710013107201018No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCTTGCCATGTAGAACCATGTC310.12312825197600985No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA290.11518449378400922No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATATTCCTTCA290.11518449378400922No Hit
ACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG260.10326885649600825No Hit
GTTGGCGGAACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT260.10326885649600825No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA260.10326885649600825No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)