FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20674
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT755836.557995549966144No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA658031.82741607816581No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC374218.100029021959948No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1770.8561478185160105No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1390.6723420721679404No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT650.31440456612169876No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT430.20799071297281613No Hit
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT380.18380574634807004No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC370.17896875302312082No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT370.17896875302312082No Hit
TTCTCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT350.1692947663732224No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG350.1692947663732224No Hit
TTCTGCAGTTTGGTGAATAGTTGATATATTGAGAGAATCTGGATGATATT340.1644577730482732No Hit
TTCTGCAGTTTGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT310.14994679307342554No Hit
TTTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT310.14994679307342554No Hit
TCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT300.14510979974847635No Hit
TGCCTCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT270.13059881977362872No Hit
TTCTGCGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT260.1257618264486795No Hit
TGCCATCTTGCCACATCTTATCGCAGGCTACCACGTCCTTCATCGCCTTC250.12092483312373029No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT240.11608783979878108No Hit
TTCTGCAGTTTGGCTAATAGTTGATATATTGAGAGAATCTGGATGATATT240.11608783979878108No Hit
TTCTGCATTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT220.10641385314888266No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC220.10641385314888266No Hit
TGCCATCTTGCCACATCTTTTCTGTTTCTGTCTGTCCCTTTCCTTTTTGG220.10641385314888266No Hit
TTCGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT210.10157685982393344No Hit
TGCCATCTTGCCACATCTCTTTTTAGTGTCCAATTTCTTTTATTTCTTTA210.10157685982393344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)