FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20674
Total Bases2.9 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG997548.24900841636838No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC641731.038986166199088No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2301.1125084647383188No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2301.1125084647383188No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA2261.0931604914385218No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1890.914191738415401No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1720.8319628518912645No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1330.6433201122182451No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1010.4885363258198704No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA990.4788623391699719No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA910.44016639257037826No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA860.41598142594563225No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA790.3821224726709877No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT490.23701267292251135No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG330.15962077972332397No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAGGATTCG300.14510979974847635No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC240.11608783979878108No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT230.11125084647383186No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT230.11125084647383186No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG220.10641385314888266No Hit
GTTGGCGGATACCCTTCCATAAAGGCCTTAACACAGAATCAAAAGATTCG220.10641385314888266No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACGGAATCAAAAGATTCG210.10157685982393344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)