FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26252
Total Bases3.8 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1033539.3684290720707No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA758528.893036721011732No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT350013.332317537711413No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC12824.88343745238458No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG5171.9693737619990856No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG3241.2341916806338564No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC640.24379094926100867No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT510.1942709126923663No Hit
TGCCATCTTGCCACATCTTCCTTCAGCAAGCAGTGGTATCAACGCAGAGT480.18284321194575653Clontech Universal Primer Mix Long (100% over 25bp)
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT340.12951394179491085No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA310.11808624104830108No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC300.11427700746609781No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT290.11046777388389456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)