FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11965
Total Bases1.7 Mbp
Sequences flagged as poor quality0
Sequence length142-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC607250.748015043877984No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC194816.28081905557877No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG153012.787296280819055No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC2281.9055578771416632No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1321.1032177183451735No Hit
GTTGGCGGATACCCTTGATGGCACAAGATGTGGCAAGATGGCATTGCCAT570.4763894692854158No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT390.325950689511074No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATAC340.2841621395737568No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT320.26744671959882993No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG310.2590890096113665No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA290.24237358963643962No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA270.22565816966151273No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA230.19222732971165898No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT230.19222732971165898No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA220.18386961972419558No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG220.18386961972419558No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT190.15879648976180527No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACAC170.1420810697868784No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAT160.13372335979941496No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA160.13372335979941496No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACGCACGCAAAATAC150.12536564981195153No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTCACACACACGCAAAATAC150.12536564981195153No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC150.12536564981195153No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC150.12536564981195153No Hit
CAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT140.1170079398244881No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC140.1170079398244881No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACGCACACGCAAAATAC140.1170079398244881No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT140.1170079398244881No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTAC140.1170079398244881No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCATTGCC130.10865022983702466No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA130.10865022983702466No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA120.10029251984956122No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACCCACGCAAAATAC120.10029251984956122No Hit
CGAATAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT120.10029251984956122No Hit
CGAAGTAGCCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT120.10029251984956122No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC120.10029251984956122No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)