FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21768
Total Bases3.1 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA588427.03050349136347No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT471421.65564130834252No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC407718.72932745314223No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA310614.268651231165014No Hit
TGCCATCTTGCCACATCTTGCTTGTGGCCCATTTCAAGTTCTATAATTTG3121.4332965821389196No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2571.1806321205439176No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1540.7074604924660052No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT740.3399485483278206No Hit
TGCCATCTTGCCACATCTGATCTGAGGTCGCGAAGGGTATCCGCCAACCA690.3169790518191841No Hit
TGCCATCTTGCCACATCTTACTTGTGGCCCATTTCAAGTTCTATAATTTG630.2894156560088203No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT540.24807056229327454No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT480.2205071664829107No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC460.21131936787945607No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT420.19294377067254684No Hit
TGCCATCTTGCCACATCTTATCTGAGGTCGCGAAGGGTATCCGCCAACCA360.16538037486218302No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT330.1515986769570011No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT290.13322307975009187No Hit
GAGGACATCTTATGGTGCACTGTAGGAGGACTCAGCAGCTGTTTTATGGA270.12403528114663727No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT260.11944138184490996No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC260.11944138184490996No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT250.11484748254318264No Hit
TGCCATCTTGCCACATCTAACTATGTCAGAAAAGTGGGGCCTACTCCTGC250.11484748254318264No Hit
TGCCATCTTGCCACATCTTAGCCTCCCAAAGTGATGGGACTACAGGCATG250.11484748254318264No Hit
TGCCATCTTGCCACATCTTGTGCCATCTTGCCCATCAAGGGTATCCGCCA250.11484748254318264No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG250.11484748254318264No Hit
TTCTGCGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT250.11484748254318264No Hit
TGCCATCTTGCCACATCTTGAAGGGTATCCGCCAACCAGTCAAGACCAAG250.11484748254318264No Hit
TTCTGCAGTTTGGTGAATAGTTGATATATTGAGAGAATCTGGATGATATT230.10565968393972804No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT220.10106578463800074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)