FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23055
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA661528.692257644762524No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA328814.261548471047494No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT16096.978963348514422No Hit
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGAAG13315.773151160268922Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGAAGC11965.187594881804381Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGTAT6943.0101930166991977Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTGCAGTGGTATCAACGCAGAGTACATGGAAGC6933.005855562784645Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGCCATCTTGCCACATCTTGCAGTGGTATCAACGCAGAGTACATGGGAAG6782.940793754066363Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGCCATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGTAT4982.160052049446975Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGAAG4471.9388418998048145Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTGCAGTGGTATCAACGCAGAGTACATGGGTAT2611.1320754716981132Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2421.0496638473216222No Hit
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGGTG2100.9108653220559532Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1740.7547169811320755No Hit
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGAAAGC1030.44675775319887223Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC910.39470830622424635No Hit
TGCCATCTTGCCACATCTTGCAGTGGTATCAACGCAGAGTACATGGGGTG910.39470830622424635Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGCCATCTTGCCACATCTTGCAGTGGTATCAACGCAGAGTACATGAAAGC700.30362177401865104Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGCCATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGGTG700.30362177401865104Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGGTA670.2906094122749946Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGAGC670.2906094122749946Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT660.2862719583604424No Hit
TGCCATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGAAGC570.247234873129473Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGTGG500.2168726957276079Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGATG500.2168726957276079Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGA480.20819778789850357Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC400.1734981565820863No Hit
TGCCATCTTGCCACATCTTGCAGTGGTATCAACGCAGAGTACATGGGGTA380.16482324875298202Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT370.16048579483842984No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT360.15614834092387767No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA360.15614834092387767Clontech Universal Primer Mix Long (96% over 25bp)
TGCCATCTTGCCACATCAAGCAGTGGTATCAACGCAGAGTACATGGGGTA350.15181088700932552Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACCAGTC340.14747343309477337No Hit
TGCCATCTTGCCACATCTTACAGTGGTATCAACGCAGAGTACATGGAAGC330.1431359791802212Clontech SMARTer II A Oligonucleotide (95% over 23bp)
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGAGA320.13879852526566905Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCAAGGGTATCCGCCAACCAGTCAAGACCAAGTCT310.1344610713511169No Hit
TGCCATCTTGCCACATCTTGCAGTGGTATCAACGCAGAGTACATGGGAGC290.12578616352201258Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TGCCATCTTGCCACATCTTACAGTGGTATCAACGCAGAGTACATGGGAAG290.12578616352201258Clontech SMARTer II A Oligonucleotide (95% over 23bp)
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT280.12144870960746042No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT270.11711125569290827No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC260.1127738017783561No Hit
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGGAA250.10843634786380395Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTAGCAGTGGTATCAACGCAGAGTACATGGGCAT250.10843634786380395Clontech SMARTer II A Oligonucleotide (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)