FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16557
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA630438.07453041009845No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA440026.574862595880894No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT273316.506613516941478No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC5403.261460409494474No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACTTGCTTCCGGGCTGA1681.0146765718427253No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG1560.9421996738539591No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT990.5979344084073202No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG810.48921906142417104No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT570.3442652654466389No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT530.32010629945038355No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT490.29594733345412816No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT390.23554991846348977No Hit
TGCCATCTTGCCACATCTTAGGACGGTGGCCATGGAAGGGTATCCGCCAA300.1811922449719152No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC300.1811922449719152No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT280.16911276197378752No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT260.15703327897565986No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT250.150993537476596No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC200.1207948299812768No Hit
AAGGTGGATGGTGTGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT190.11475508848221297No Hit
AAGGTGGATGGGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT170.10267560548408527No Hit

[OK]Adapter Content

Adapter graph