FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16090
Total Bases2.3 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC689642.85891858297079No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC304818.94344313238036No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG15139.403356121814792No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC3842.386575512740833No Hit
GTTGGCGGATACCCTTCCATTCACCAAAGATGATTGCTTTAATAGTTGTT2101.305158483530143No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC2061.2802983219390927No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT900.5593536357986327No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCATT580.36047234307022996No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT540.3356121814791796No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAACAC520.32318210068365444No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG500.3107520198881293No Hit
GTTGGCGGATACCCTTCCCCTTCCGGAGATGTGGCAAGATGGCATTGCCA500.3107520198881293No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG410.254816656308266No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT350.2175264139216905No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT350.2175264139216905No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA340.2113113735239279No Hit
GTTGGCGGATACCCTTCCACCGCGCTGGGGAAAAGATGTGGCAAGATGGC340.2113113735239279No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA320.19888129272840274No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA290.18023617153511498No Hit
GTTGGCGGATACCCTTCCTCGCGCCAGGTAGATGTGGCAAGATGGCATTG290.18023617153511498No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT280.1740211311373524No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA260.16159105034182722No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCATTGCC260.16159105034182722No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA260.16159105034182722No Hit
CGAAGTAGACCAGCGGCATCCCGGCCCCACCCTGGACCTTAGCTCAGCAC260.16159105034182722No Hit
GTTGGCGGATACCCTTCCCCTTCCGAAGATGTGGCAAGATGGCATTGCCA250.15537600994406464No Hit
CGAAGTGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT240.14916096954630206No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT230.14294592914853949No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC220.13673088875077688No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACGCACACGCAAAATAC210.13051584835301427No Hit
CGAAGTAACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT210.13051584835301427No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTAC200.1243008079552517No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC190.11808576755748912Clontech Universal Primer Mix Long (96% over 26bp)
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCATT190.11808576755748912No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC180.11187072715972653No Hit
CGAAGTAGACCGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT170.10565568676196395No Hit
TACCTCAGTTTGCCCCCATGACAGGGATAAAGTATAGTTTTTGTTGCTGG170.10565568676196395No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC170.10565568676196395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)