FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18563
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA566530.517696493023756No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT379420.43850670689005No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA313916.90998222270107No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC217511.716856111619888No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG4032.1709852933254323No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2401.2928944674890912No Hit
TGCCATCTTGCCACATCTGTGACTGTAGAGTGAGGAATATCTATACAAAA1320.7110919571190002No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT920.4956095458708183No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT410.22086947152938644No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT380.2047082906857728No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACCAGTC380.2047082906857728No Hit
TGCCATCTTGCCACATCTACCGGGAAGGGTATCCGCCAACCAGTCAAGAC370.19932123040456823No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC350.18854710984215914No Hit
TGCCATCTTGCCACATCTTAGAAGGGTATCCGCCAACCAGTCAAGACCAA330.17777298927975005No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT320.1723859289985455No Hit
TGCCATCTTGCCACATCGTACCAGCTGCTAATGCTGGCTCAGCACAAGGG320.1723859289985455No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC320.1723859289985455No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT310.16699886871734093No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT300.1616118084361364No Hit
TGCCATCTTGCCACATCTAATTGCTTTCATAAGCAGTGGTATCAACGCAG290.15622474815493184Clontech Universal Primer Mix Long (95% over 21bp)
TGCCATCTTGCCACATCTTACCAGCTGCTAATGCTGGCTCAGCACAAGGG290.15622474815493184No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC290.15622474815493184No Hit
TGCCATCTTGCCACATCTTAGTCACTTTCCTTCCTTGCTTCCGGGCTTTC280.1508376878737273No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT270.14545062759252278No Hit
TGCCATCTTGCCACATCTCAAGCCAAGCAGTGGTATCAACGCAGAGTACA260.1400635673113182Clontech Universal Primer Mix Long (96% over 26bp)
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG250.13467650703011366No Hit
TGCCATCTTGCCACATCTTGATGGGGGAATTCTTTCTGCAGTAGGAAGGG240.12928944674890913No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG230.12390238646770457Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCTGAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT220.11851532618650004No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACACAGAAGGGTATCCGCCA210.11312826590529547No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG200.10774120562409092No Hit
TGCCATCTTGCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT200.10774120562409092No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGCTTCCCAGTCCACTA190.1023541453428864No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)