FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17597
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA750042.62090128999262No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA309117.565494118315623No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC216812.320281866227198No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT176310.018753196567596No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG3071.7446155594703643No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2051.1649713019264647No Hit
TGCCATCTTGCCACATCTGCATCTTGACAGGACGGTGGCCATGGAAGGGT1210.6876172074785475No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT710.40347786554526344No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT500.2841393419332841No Hit
TGCCATCTTGCCACATCTGCATCTTGACAGGACGGTGGCTATGGAAGGGT480.27277376825595273No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC450.25572540773995567No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT440.25004262090129No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT310.17616639199863612No Hit
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT310.17616639199863612No Hit
TGCCATCTTGCCACATCTTATATGGAAGGGTATCCGCCAACCAGTCAAGA300.17048360515997044No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT270.1534352446439734No Hit
AAGGTGGTGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT260.1477524578053077No Hit
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT260.1477524578053077No Hit
GAGGACATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGG230.13070409728931068No Hit
AAGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT210.11933852361197932No Hit
TGCCATCTTGCCACATCTTAGATCAAATGCCATCTCCTCAGGAATGTCTT210.11933852361197932No Hit
AAGGTGGATGGTGATGTGGTCCCACACATCAGCGCTGCTGGCGCTGGTGA210.11933852361197932No Hit
AAGGTGGATGGTATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT190.10797294993464795No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGCCCACTA190.10797294993464795No Hit
TTCTGCAGTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATATT180.10229016309598227No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACCAGTCAAGACCAAGTCT180.10229016309598227No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC180.10229016309598227No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTCCCCAGTCCACTA180.10229016309598227No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)