FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13936
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC614144.06572904707233No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG302221.684845005740527No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC10647.634902411021814No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC3492.504305396096441No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC2061.4781859931113663No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA760.5453501722158438No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA660.473593570608496No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATCAGATGTGGCAAGATGGCATT640.4592422502870264No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT580.41618828932261764No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA550.3946613088404133No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG520.3731343283582089No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA490.3516073478760046No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT350.2511481056257176No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC340.24397244546498276Clontech Universal Primer Mix Long (96% over 26bp)
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA300.2152698048220436No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCATTGCCATGTAGAACCAT300.2152698048220436No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG290.20809414466130882No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA290.20809414466130882No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA290.20809414466130882No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT280.20091848450057406No Hit
GTTGGCGGATACCCTTGTAGTCCTGTCTCTTATACACATCTCCGAGCCCA270.19374282433983928No Hit
GTTGGCGGATACCCTTGAGATGTGGCAAGATGGCATTGCCATGTAGAACC270.19374282433983928No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA250.1793915040183697No Hit
GTTGGCGGATACCCTTCCCTTTAAACACTGGTCTTCCAAAACGTATTCAT250.1793915040183697No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA240.1722158438576349No Hit
GTTGGCGGATACCCTTCGCGACCTCAGATAAGATGTGGCAAGATGGCATT240.1722158438576349No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATAC220.1578645235361653No Hit
GTTGGCGGATACCCTTGGCAACTAGTGCATTTGGACTGGAACTACTTGGG220.1578645235361653No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT200.14351320321469577No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC180.1291618828932262No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC180.1291618828932262No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT170.12198622273249138No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTAC170.12198622273249138No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC150.1076349024110218No Hit
GTTGGCGGATACCCTTCCATCCTGTTCCTCAGGAGCCCAGCAGGGGTGCA150.1076349024110218No Hit
GTTGGCGGATACCCTTCTCTTAACATTTAAGCAAGCTGTTTTTATAGCAG140.10045924225028703No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCTTGCCATGTAGAACCATGTC140.10045924225028703No Hit
TACCTCAGTTTGCCCCCATGATCGCTGTGGGGGATGCTTATCTATGCCCC140.10045924225028703No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCATTGCC140.10045924225028703No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)