FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18981
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC907747.821505716242555No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG482825.435962278067542No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC10965.774195247879459No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1260.6638217164532955No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1140.6006006006006006No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1100.5795268953163689No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA950.5005005005005005No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT770.4056688267214583No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC720.3793266951161688No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA700.368789842474053No Hit
GTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA660.3477161371898214No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA590.3108371529424161No Hit
GTTGGCGGATACCCTTCCCAGAGGGTGGAGAAGATGTGGCAAGATGGCAT560.2950318739792424No Hit
TACCTCAGTTTGCCCCCATGGGCTGGGAGGGAGGGCGGGGACTGCCAGGT550.28976344765818446No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA500.26342131605289504No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA480.2528844634107792No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT400.21073705284231603No Hit
GTTGGCGGATACCCTTCCAGAGGGTGGAGAAGATGTGGCAAGATGGCATT370.1949317738791423No Hit
GTGTCGCTACCTCAGTTTGTCCCCATGTCCCTTACACACACGCAAAATAC300.158052789631737No Hit
GTTGGCGGATACCCTTGCATAAAGGCTTTAACACAGAATCAAAAGATTCG300.158052789631737No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGCCCCTTACACACACGCAAAATAC290.1527843633106791No Hit
GTGTCGCTACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACT290.1527843633106791No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT290.1527843633106791No Hit
GTTGGCGGAACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA280.1475159369896212No Hit
GTTGGCGGATCCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA270.1422475106685633No Hit
GTTGGCGGATACCCTTCCATAAGCAGCAGCCACTGTTCGTCTCTGGAGGA270.1422475106685633No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAGTAC240.1264422317053896No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATAC240.1264422317053896No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCATTGCC240.1264422317053896No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA220.1159053790632738No Hit
GTGTCGCTACCTCAGTTTGCCCCCACGTCCCTTACACACACGCAAAATAC190.10010010010010009No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)