FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33634
Total Bases4.8 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1210635.993340072545635No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA461513.721234465124576No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA32099.540940714752928No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC27338.125706130701078No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT23937.114824284949753No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAA9212.738300529226378Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG8512.5301777962775764No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTA6711.9950050544092288Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG5961.772016411964084No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGTA2820.8384372955937445Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGAA1830.5440922875661532Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC1150.34191591841588864No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGGT1060.3151572813224713Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAT900.26758637093417376Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGTG780.23190818814295058Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT740.22001546054587617No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC640.19028364155319022No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAG550.16352500445977286Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGAAG550.16352500445977286Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCCATCTTGCCACATCTTGTCTCAAACTGTTGGGCTGAAGCAGTTCTCC550.16352500445977286No Hit
TGCCATCTTGCCACATCTTAGCAGTGGTATCAACGCAGAGTACATGGGAC490.14568591306416126Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT450.13379318546708688No Hit
TGCCATCTTGCCACATCTAAGCAGTGGTATCAACGCAGAGTACATGGGGT440.13082000356781828Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGATGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT430.1278468216685497No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT420.12487363976928108No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC380.1129809121722067No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT350.10406136647440091No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT340.1010881845751323No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)