FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22897
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA808635.31467004411058No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT560624.483556797833778No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT19328.437786609599511No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACCAGTCA10024.376119142245709No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG9594.188321614185265No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG6282.742717386557191No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG3751.637769140062017No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC3541.5460540682185437No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAGAAGGGTATCCGCCAAC1690.7380879591212822No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATCCGCCAACCAGTC1670.7293531903742848No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAAGGGTATCCGCCAACCA1210.5284535091933441No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC910.3974319779883827No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAGGAAGGGTATCCGCCAA890.38869720924138534No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG590.257675678036424Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC550.24020614054242914No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCCGCCAACACAGAA490.21400183430143688No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGGGAAGGGTATCCGCCAAC470.20526706555443944No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT400.17469537493994847No Hit
TGCCATCTTGCCACATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAA400.17469537493994847No Hit
TGCCATCTTGCCACATCTACCTGGCGCGAGGGAGGAGGGTATCCGCCAAC290.12665414683146264No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATTTGGTTTCCCAGTC280.12228676245796392No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAGGGTATCGCCAACCAGTCAA270.11791937808446522No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATCCGCCAACCAGTC270.11791937808446522No Hit
TGCCATCTTGCCACATCTTCCTCCCCAAGCAGTGGTATCAACGCAGAGTA260.1135519937109665Clontech Universal Primer Mix Long (96% over 25bp)
TGCCCTCTCTGCCTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTAT230.10044984059047037No Hit
AAGGTGGATGGTGATGTGGTCCCACACATCAGCGCTGCTGGCGCTGGTGA230.10044984059047037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)