FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22897
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC790134.50670393501332No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA528323.072891645193693No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG20629.005546578154343No Hit
GTTGGCGGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCA9734.249464995414246No Hit
GTTGGCGGATACCCTTCCATTCACCAAAGATGATTGCTTTAATAGTTGTT8063.520111805039962No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC6212.7121456959427No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC3711.6202996025680219No Hit
GTTGGCGGATACCCTTCCCATTTCAGTGCACCATAAGATGTCCTCTTGCC1620.7075162685067913No Hit
GTTGGCGGATACCCTTCTCCCTCGCGCCAGGTAGATGTGGCAAGATGGCA1480.6463728872778093No Hit
GTTGGCGGATACCCTTCCCTCGCGCCAGGTAGATGTGGCAAGATGGCATT1210.5284535091933441No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG890.38869720924138534No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT880.38432982486788664No Hit
GTTGGCGGATACCCTTCCTCCCTCGCGCCAGGTAGATGTGGCAAGATGGC820.35812551862689435No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG550.24020614054242914No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG500.21836921867493556No Hit
GTTGGCGGATACCCTTCCCCCTCGCGCCAGGTAGATGTGGCAAGATGGCA470.20526706555443944No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA460.20089968118094073No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG430.18779752806044459No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA420.18343014368694588No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT400.17469537493994847No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA400.17469537493994847No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA390.17032799056644973No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG380.16596060619295105No Hit
GTTGGCGGATACCCTTCCATCGTCCACCGCAAATGCTTCTAGGCGGACTA380.16596060619295105No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG380.16596060619295105No Hit
TTGGCGGATACCCTTCCATTCACCAAAGATGATTGCTTTAATAGTTGTTA300.13102153120496135No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG290.12665414683146264No Hit
GTTGGCGGATACCCTCCTCCCTCGCGCCAGGTAGATGTGGCAAGATGGCA280.12228676245796392No Hit
GTTGGCGATACCCTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCCAT270.11791937808446522No Hit
GTTGGCGGATACCCTTTCCATTTCAGTGCACCATAAGATGTCCTCTTGCC260.1135519937109665No Hit
GTGTCGCTACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACT230.10044984059047037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)