FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46186
Total Bases6.6 Mbp
Sequences flagged as poor quality0
Sequence length140-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT2243848.58182133113931No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA765916.5829472134413No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTCAGCCACCTGCTTCCGGGCTGA467710.126445243147273No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT30196.53661282639761No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC28916.259472567444681No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG3870.8379162516779978No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG2590.5607759927250682No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG2170.4698393452561382No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT1310.28363573377213874No Hit
AGGTGGATGGTGATGTGGTCTTCAATTCAGCCACCTGCTTCCGGGCTGAT1040.2251764603992552No Hit
GAGGACATCTTATGGTGCACTGAAATGGGAAGGGTATTTGGTTTCCCAGT630.13640497120339495No Hit
AAGGTGGATGTGATGTGGTCTTCAATTCAGCCACCTGCTTCCGGGCTGAT590.1277443381111159No Hit
AAGGTGATGGTGATGTGGTCTTCAATTCAGCCACCTGCTTCCGGGCTGAT590.1277443381111159No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC590.1277443381111159No Hit
GAGGACATCTTATGGTGCACTGAAATGGAGAGGGTATTTGGTTTCCCAGT570.1234140215649764No Hit
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGCTTCCCAGT540.11691854674576713No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC520.1125882301996276No Hit
GAGGACATCTTATGGTGCACTGAAGTGGAAAGGGTATTTGGTTTCCCAGT490.1060927553804183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)