FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41416
Total Bases5.9 Mbp
Sequences flagged as poor quality0
Sequence length35-145
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA1917646.30094649410856No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC820619.813598609233146No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG41369.986478655592041No Hit
CGAAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCAC33278.033127293799499No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC2330.5625845084025497No Hit
TACTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1890.45634537376859186No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC1590.3839096001545297No Hit
TACCTCATTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1510.3645933938574464No Hit
TACCTCAGTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1400.33803361019895695No Hit
TACCTAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1160.28008499130770714No Hit
TACCTCAGTTTGCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG1110.26801236237203013No Hit
TTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA1050.25352520764921765No Hit
GTTGGCGGATACCCTTCCCTGCAACAGAAAGACTGTAGCCTAGAATCTCT910.219721846629322No Hit
TACCTCGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG840.20282016611937415No Hit
GTTGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA750.1810894340351555No Hit
GTTGGCGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA720.17384585667374927No Hit
GTTGGCGGTACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA680.16418775352520765No Hit
TCCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG660.15935870195093682No Hit
TACCCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCAG640.15452965037666602No Hit
CGAAGTAGACAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT600.1448715472281244No Hit
GTTGGCGGATACCCATGTCCCTTACACACACGCAAAATACTCCTTCAGCG550.13279891829244736No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACCCCTTCA510.12314081514390574No Hit
GTTGGCGGATACCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA500.12072628935677034No Hit
TACCTCAGTTTGCCCCCATGTCCCCTACACACACGCAAAATACTCCTTCA500.12072628935677034No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACACAAAATACTCCTTCA480.11589723778249951No Hit
CGAGTAGACCAGCGGCATCTCGGCCCCACCCTGGACCTTAGCTCAGCACT470.11348271199536411No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG470.11348271199536411No Hit
GTTGGGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCGA450.1086536604210933No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT450.1086536604210933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)