FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30038
Total Bases4.3 Mbp
Sequences flagged as poor quality0
Sequence length141-145
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT1165138.78753578800186No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA636221.1798388707637No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC327310.896198149011251No Hit
AAGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGA304710.14381783074772No Hit
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG8892.959584526266729No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG5801.9308875424462346No Hit
TGCCATCTTGCCACATCTTATTTAACAACTATTAAAGCAATCATCTTTGG1680.5592915640189094No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT1510.5026965843265198No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC1020.33956987815433787No Hit
TGCCATCTTGCCACATCTTCGTCAGGAGGGTTGGATTCATCATCTAGCCA850.2829748984619482No Hit
AGGTGGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT790.26300019974698713No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC610.203076103602104No Hit
TGCCATCTTGCCACATCTTAGAACATTTTGGTGCTAGCCTGGCAAACTAT590.196417870697117No Hit
TGCCATCTTGCCACATCTGACGCTGCCGACGGAAGGGTATCCGCCAACCA520.17311405552966244No Hit
TGCCATCTTGCCACATCTTAGCGAGCCCGCCGACACGCCGAGCAGGAGAG450.14981024036220789No Hit
AAGGTGATGGTGATGTGGTCTTCAATTTAGCCACCTGCTTCCGGGCTGAT410.13649377455223383No Hit
TGCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTCT400.13316465809974032No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT400.13316465809974032No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)