FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758
Total Bases254.7 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG48327.474402730375424No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG27515.642775881683733No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACCAGTCAA1428.0773606370876No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC794.493742889647327No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC543.0716723549488054No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACCAGTCAAGACCAAGTCT442.502844141069397No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT261.4789533560864618No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG251.422070534698521Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGG201.1376564277588168Clontech Universal Primer Mix Long (96% over 27bp)
GAGGACATCTTATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGT201.1376564277588168No Hit
GAGGACATCTTATGGTGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCT191.0807736063708762No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACACAGAAG181.023890784982935No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACCAGTC170.9670079635949944No Hit
TGCCATCTTGCCACATCTTATGCCATCAAGGGTATCCGCCAACCAGTCAA150.8532423208191127No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG150.8532423208191127No Hit
TTCTGCAGTTTGGCTGAATAGTTGATATATTGAGAGAATCTGGATGATAT120.6825938566552902No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACACAGAAGGGTATCCGCCA120.6825938566552902No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC110.6257110352673493No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA110.6257110352673493No Hit
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG100.5688282138794084Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCAAGGGTATCCGCCAACACAGAAGGGTATCCGCC90.5119453924914675No Hit
GAGGACATCTTATGGTGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCT90.5119453924914675No Hit
TGCCATCTTGCCACATCTTAGAAGTTTAGTTGAAATCATTGAACATGGTC70.39817974971558584No Hit
AAGGTGGATGGTGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTC60.3412969283276451No Hit
GAGGACATCTTATGGGGGCAAACGAGGTACAGTCAAGACCAAGTCTCTGC60.3412969283276451No Hit
TGCCATCTTGCCACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCA60.3412969283276451No Hit
TGCCATCTTGCCACATCTTAGAAGGGTATCCGCCAACCAGTCAAGACCAA50.2844141069397042No Hit
TGCCATCTTGCCACATCTTAGGGTATCCGCCAACCAGTCAAGACCAAGTC50.2844141069397042No Hit
TGCCATCTTGCCACATCTTGGGTATCCGCCAACCAGTCAAGACCAAGTCT50.2844141069397042No Hit
TGCCATCTTGCCACATCTGTTCTTTGAAGATGGAAGGGTATCCGCCAACC50.2844141069397042No Hit
GAGGACATCTTATGGGGGCAAACTGAGTACAGTCAAGACCAAGTCTCTGC40.22753128555176336No Hit
GAGGACATCTTATGGTGGCAAATGAGGTACAGTCAAGACCAAGTCTCTGC40.22753128555176336No Hit
GAGGACATCTTATGGTGGCAAACGAGGTACAGTCAAGACCAAGTCTCTGC40.22753128555176336No Hit
AAGGTGGATGGTGATGTCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTC40.22753128555176336No Hit
TGCCATCTTGCCACATCTGGGTATCCGCCAACCAGTCAAGACCAAGTCTC40.22753128555176336No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACAGTCAAG40.22753128555176336No Hit
GAGGACATCTTATGGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCT40.22753128555176336No Hit
TGCCATCTTGCCACATCTGGGGTATCCGCCAACACAGAAGGGTATCCGCC40.22753128555176336No Hit
GAGGACATCTTATGGGGGCAAATGAGGTACAGTCAAGACCAAGTCTCTGC40.22753128555176336No Hit
TGCCATCTTGCCACATCTTTCCACTTGGACAACCATCGCCAAGCAGTGGT30.17064846416382254No Hit
GAGGACATCTTATGGTGCACGAAGCAGTGGTATCAACGCAGAGTACATGG30.17064846416382254Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGCAAACTGAGTACAGTCAAGACCAAGTCTCTGCT30.17064846416382254No Hit
GAGGACATCTTATGGTGCACTAAAGCAGTGGTATCAACGCAGAGTACATG30.17064846416382254Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTGGGTATCCGCCAACACAGAAGGGTATCCGCC30.17064846416382254No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACCAGTCAAGACCAAGTCTC30.17064846416382254No Hit
TGCCATCTTGCCACATCTCGTGCCATCAAGGGTATCCGCCAACCAGTCAA30.17064846416382254No Hit
TGCCATCTTGCCACATCTTAGGTATCCGCCAACCAGTCAAGACCAAGTCT30.17064846416382254No Hit
GAGGACATCTTATGGTGCACACTGAGGTACAGTCAAGACCAAGTCTCTGC30.17064846416382254No Hit
TGCCATCTTGCCACATCTAGGGTATCCGCCAACCAGTCAAGACCAAGTCT30.17064846416382254No Hit
GAGGACATCTTATGGTGCACTGAGGTACAGTCAAGACCAAGTCTCTGCTA30.17064846416382254No Hit
GAGGACATCTTATGGGGGCAAACTAGGTACAGTCAAGACCAAGTCTCTGC30.17064846416382254No Hit
TGCCATCTTGCCACATCTGGAAGGGTATCCGCCAACCAGTCAAGACCAAG30.17064846416382254No Hit
GAGGACATCTTATGGGGGCAACTGAGGTACAGTCAAGACCAAGTCTCTGC30.17064846416382254No Hit
TGCCATCTTGCCACATCTGGAGGGTATCCGCCAACCAGTCAAGACCAAGT30.17064846416382254No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACACAGGGT30.17064846416382254No Hit
GAGGACATCTTATGGTGGCAAACTGAGTACAGTCAAGACCAAGTCTCTGC30.17064846416382254No Hit
TGCCATCTTGCCACATCTTGTGCCATCATGGGTATCCGCCAACACAGAAG20.11376564277588168No Hit
TGCCATCTTGCCACATCTTGTGCCATCATGGGTATCCGCCAACCAGTCAA20.11376564277588168No Hit
TGCCATCTTGCCACATCTTATGCCATCAAGGGTATCCGCCAACACAGAAG20.11376564277588168No Hit
TGCCATCTTGCCCATCTTGTGCCATCAAGGGTATCCGCCAACCAGTCAAG20.11376564277588168No Hit
TGCCATCTTGCCACATCTCATGGGGGCAAACTGAGGTACAGTCAAGACCA20.11376564277588168No Hit
GAGGACATCTTATGGTGCACTGGGGGCAAACTGAGGTACAGTCAAGACCA20.11376564277588168No Hit
TGCCATCTTGCCAATCTTGTGCCATCAAGGGTATCCGCCAACCAGTCAAG20.11376564277588168No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTACCGCCAACCAGTCAAG20.11376564277588168No Hit
TGCCATCTTGCCACATCAGGGTATCCGCCAACCAGTCAAGACCAAGTCTC20.11376564277588168No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCAACCAGTCAAG20.11376564277588168No Hit
TGCCCTCTCTGCCTTTTCAAAGCAGTGGTATCAACGCAGAGTACATGGGT20.11376564277588168Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGGTGGATGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTA20.11376564277588168No Hit
GAGGACATCTTATGGTGGCAAACTGGGGTACAGTCAAGACCAAGTCTCTG20.11376564277588168No Hit
TGCCATCTTGCCACATCTGGGGGTATCCGCCAACCAGTCAAGACCAAGTC20.11376564277588168No Hit
GAGGACATCTTAGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC20.11376564277588168No Hit
GAGGACATCTTATGGGGACAAACTGAGGTACAGTCAAGACCAAGTCTCTG20.11376564277588168No Hit
TGCCATCTTGCCACATCTGTTCTGGAGATGGAAGGGTATCCGCCAACCAG20.11376564277588168No Hit
TGCCATCTTGCCACATCTTCGCCCAAGCAGTGGTATCAACGCAGAGTACA20.11376564277588168Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGTGGCAAGCTGAGGTACAGTCAAGACCAAGTCTCTG20.11376564277588168No Hit
GAGGACATCTTATGGTGACAAACTGAGGTACAGTCAAGACCAAGTCTCTG20.11376564277588168No Hit
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCGCCAACCAGTCA20.11376564277588168No Hit
GAGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC20.11376564277588168No Hit
TGCCATCTTGCCACATCTCAAAAGGGTATCCGCCAACCAGTCAAGACCAA20.11376564277588168No Hit
TGCCATCTTGCCACATCTTAGTATCCGCCAACACAGAAGGGTATCCGCCA20.11376564277588168No Hit
TGCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACCAGTCAAG20.11376564277588168No Hit
TGCCATCTTGCCACATCTTAGCAAGGGTATCCGCCAACCAGTCAAGACCA20.11376564277588168No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAAGGTATCCGCCACAGTCAAGA20.11376564277588168No Hit
GAGGACATCTTATGGTGCATGGGGGCAAACTGAGGTACAGTCAAGACCAA20.11376564277588168No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCACCAGTCAAGACCAAGTCTC20.11376564277588168No Hit
TGCCATCTTGCCACATCTGGGGTATCCGCCAACCAGTCAAGACCAAGTCT20.11376564277588168No Hit
TGCCATCTTGCCACATCTTAGGGAAGGGTATCCGCCAACCAGTCAAGACC20.11376564277588168No Hit
TGCCATCTTGCCACATCTTAGGTATCCGCCAACACAGAAGGGTATCCGCC20.11376564277588168No Hit
GAGGACATCTTATGGTGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTC20.11376564277588168No Hit
TGCCATCTTGCCACATCTTGTGCCATCAAGGGTATCCGCCAACACGGAAG20.11376564277588168No Hit
GAGGACGTCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG20.11376564277588168No Hit
TGCCATCTTGCCACATCTTAGTATCCGCCAACCAGTCAAGACCAAGTCTC20.11376564277588168No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)