FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758
Total Bases254.7 kbp
Sequences flagged as poor quality0
Sequence length35-145
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC47326.905574516496017No Hit
TACCTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC27515.642775881683733No Hit
GTTGGCGGATACCCTTGATGGCACAAGATGTGGCAAGATGGCATTGCCAT1377.792946530147896No Hit
TACCTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG764.323094425483505No Hit
TACCTCAGTTTGCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG522.9579067121729237No Hit
GTTGGCGGATACCCTTGATGTGGCAAGATGGCATTGCCATGTAGAACCAT442.502844141069397No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGGCGGAT412.3321956769055743No Hit
CGAAGTAGACCACATCACCATCCACCTTTTGCCATGTAGAACCATGTCGT241.3651877133105803No Hit
GTTGGCGGATACCCTTCCATAAAGGCTTTAACACAGAATCAAAAGATTCG191.0807736063708762No Hit
TACCTCAGTTTGCCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGT191.0807736063708762No Hit
TACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATACTCCTTCA181.023890784982935No Hit
GTTGGCGGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCATTGCC160.9101251422070534No Hit
TACCTCAGTTTGCCCCCATGAGATGTCCTCTTGCCATGTAGAACCATGTC150.8532423208191127No Hit
GTTGGCGGATACCCTTGATGGCATAAGATGTGGCAAGATGGCATTGCCAT140.7963594994311717No Hit
CGAAGTAGACCAGCGGCATCCACCTTTTGCCATGTAGAACCATGTCGTCA120.6825938566552902No Hit
GTGTCGCTACCTCAGTTTGCCCCCATGTCCCTTACACACACGCAAAATAC110.6257110352673493No Hit
TACCTCAGTTTGCCCCCACCATAAGATGTCCTCTTGCCATGTAGAACCAT90.5119453924914675No Hit
CGAAGTAGACCAGCGGCATCACCATCCACCTTTTGCCATGTAGAACCATG60.3412969283276451No Hit
TACCTCAGTTTGCCCCCATAAGATGTGGCAAGATGGCATTGCCATGTAGA60.3412969283276451No Hit
GTTGGCGGATACCCAAGATGTGGCAAGATGGCATTGCCATGTAGAACCAT50.2844141069397042No Hit
GTTGGCGGATACCCTAAGATGTGGCAAGATGGCATTGCCATGTAGAACCA50.2844141069397042No Hit
GTTGGCGGATACCCTTCTAAGATGTGGCAAGATGGCATTGCCATGTAGAA50.2844141069397042No Hit
TACCTCGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG50.2844141069397042No Hit
GTTGGCGGATACCCAGATGTGGCAAGATGGCATTGCCATGTAGAACCATG40.22753128555176336No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACTGCTTCAGTGCACC40.22753128555176336Clontech SMART CDS Primer II A (96% over 26bp)
TACTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG40.22753128555176336No Hit
GTTGGCGGATACCCTTCCATCTTCAAAGAACAGATGTGGCAAGATGGCAT40.22753128555176336No Hit
TACCTCATTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG40.22753128555176336No Hit
TTGGCGGATACCCTTGATGGCACAAGATGTGGCAAGATGGCATTGCCATG40.22753128555176336No Hit
TACCTCGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG40.22753128555176336No Hit
TACCTCAGTTTGCCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGT40.22753128555176336No Hit
CGAAGTAGACCAGCGACATCACCATCCACCTTTTGCCATGTAGAACCATG40.22753128555176336No Hit
TACCTCATTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG40.22753128555176336No Hit
GTTGGCGGATACCCTCCAGATGTGGCAAGATGGCATTGCCATGTAGAACC30.17064846416382254No Hit
GTTGGCGGATACCCTAGATGTGGCAAGATGGCATTGCCATGTAGAACCAT30.17064846416382254No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGATGGCGGAT30.17064846416382254No Hit
GTTGGCGGATACCCTTCCAGATGTGGCAAGATGGCATTGCCATGTAGAAC30.17064846416382254No Hit
GTTGGCGGATACCTAAGATGTGGCAAGATGGCATTGCCATGTAGAACCAT30.17064846416382254No Hit
TACTCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG30.17064846416382254No Hit
TACCTCAGTGTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG30.17064846416382254No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCAAGTC30.17064846416382254No Hit
TACCTCAGTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCGTC30.17064846416382254No Hit
TACCTCAGTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG30.17064846416382254No Hit
TACCTAGTTTGCCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTCG30.17064846416382254No Hit
GTTGGCGGATACCCTTCCATGTTGGCGGATACCCTTCTGTGTTGGCGGAT30.17064846416382254No Hit
TACTCAGTTTGCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTCGT30.17064846416382254No Hit
GTTGGCGGATACCCTTGATGGCACGAGATGTGGCAAGATGGCATTGCCAT20.11376564277588168No Hit
TACCCCAGTTTGCCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC20.11376564277588168No Hit
GTGGCGGATACCCTTGATGTGGCAAGATGGCATTGCCATGTAGAACCATG20.11376564277588168No Hit
TACCTCAGTTTGCCCCCAGTGCACCATAAGATGTCCTCTTGCCATGTAGA20.11376564277588168No Hit
GTTGGCGGATACTAAGATGTGGCAAGATGGCATTGCCATGTAGAACCATG20.11376564277588168No Hit
CGAAGTAGACCACATCACATCCACCTTTTGCCATGTAGAACCATGTCGTC20.11376564277588168No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTGTGTGTTGGCGGAT20.11376564277588168No Hit
TACCTCAGTTTGCCCCACCATAAGATGTCCTCTTGCCATGTAGAACCATG20.11376564277588168No Hit
TGGCGGATACCTTTGATGGCACAAGATGTGGCAAGATGGCATTGCCATGT20.11376564277588168No Hit
TACCTCAGTTTGCCACCATAAGACGTCCTCTTGCCATGTAGAACCATGTC20.11376564277588168No Hit
GTTGGCGGATACCCTTCCGTGTTGGCGGATACCCTTGATGGCACAAGATG20.11376564277588168No Hit
GTTGGCGGATACCCTTGATGGCACAAGATGGGCAAGATGGCATTGCCATG20.11376564277588168No Hit
TACCTCAGTTTGCCACCATAAGATGTCTCTTGCCATGTAGAACCATGTCG20.11376564277588168No Hit
GTTGGCGGATACCCTTTTGAGATGTGGCAAGATGGCATTGCCATGTAGAA20.11376564277588168No Hit
TACCTCAGTTTGCCCCCTAAGATGTCCTCTTGCCATGTAGAACCATGTCG20.11376564277588168No Hit
GTTGGCGGATACCCATGTACTCTGCGTTGATACCACCCCATGTACTCTGC20.11376564277588168Clontech SMART CDS Primer II A (95% over 21bp)
TACCTCAGTTTGTCCCCATAAGATGTCCTCTTGCCATGTAGAACCATGTC20.11376564277588168No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTCTGTGTTGTCGGAT20.11376564277588168No Hit
GTTGGCGGATACCAAGATGTGGCAAGATGGCATTGCCATGTAGAACCATG20.11376564277588168No Hit
GTTGGCGGATACCCTTGATGGCACAAGATGTGGCAAGATGCATTGCCATG20.11376564277588168No Hit
GTTGGCGGATACCCTTCCATCTCCAGAACAGATGTGGCAAGATGGCATTG20.11376564277588168No Hit
GTTGGCGGATACCCCCAGATGTGGCAAGATGGCATTGCCATGTAGAACCA20.11376564277588168No Hit
GTTGGCGGATACCCATGATGGCACAAGATGTGGCAAGATGGCATTGCCAT20.11376564277588168No Hit
GTTGGCGGATACCCTTCTGTGTTGGCGGATACCCTTGATGTGGCAAGATG20.11376564277588168No Hit
GTTGGCGATACCCTTCCATCTTCAACAGATGTGGCAAGATGGCATTGCCA20.11376564277588168No Hit
TACCTCAGTTTGTCACCATAAGATGTCCTCTTGCCATGTAGAACCATGTC20.11376564277588168No Hit
TACCTCAGTTTGCCCCCATGCACCATAAGATGTCCTCTTGCCATGTAGAA20.11376564277588168No Hit
GTTGGCGGATACCCCAGATGTGGCAAGATGGCATTGCCATGTAGAACCAT20.11376564277588168No Hit
GTTGCGGATACCCTTGATGGCACAAGATGTGGCAAGATGGCATTGCCATG20.11376564277588168No Hit
TACCTCAGTTTGCCCCCATGAGATGTGGCAAGATGGCATTGCCATGTAGA20.11376564277588168No Hit
GTTGGCGGATACCCTTCCCTAAGATGTGGCAAGATGGCATTGCCATGTAG20.11376564277588168No Hit
TACCTCAGTTTGCCCCCATAAGATGTCCTCTTTCCATGTAGAACCATGTC20.11376564277588168No Hit
GTTGGCGGATACCCTTGATGGCACAAGATTGGCAAGATGGCATTGCCATG20.11376564277588168No Hit
GTTGGCGGATACCCTGTGTTGGCGGATACCCTGTGTTGGCGGATACCCTG20.11376564277588168No Hit
GTTGGCGGTACCCTTGATGGCACAAGATGTGGCAAGATGGCATTGCCATG20.11376564277588168No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)