FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008154289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008154289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094
Total Bases158.5 kbp
Sequences flagged as poor quality0
Sequence length141-145
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGACATCTTATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG29827.239488117001827No Hit
GAGGACATCTTATGGTGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG20618.82998171846435No Hit
GAGGACATCTTATGGTGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC565.118829981718465No Hit
AAGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCT534.844606946983546No Hit
GAGGACATCTTATGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTGC393.5648994515539303No Hit
AAGGTGGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTAC282.5594149908592323No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACCAGTCAAGACCAAGTCT222.010968921389397No Hit
AAGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGG171.5539305301645339Clontech Universal Primer Mix Long (96% over 27bp)
TGCCCTCTCTGCCTTTTCTCAAGCAGTGGTATCAACGCAGAGTACATGGG171.5539305301645339Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTGTTGAAGATGGAAGGGTATCCGCCAACCAGTC141.2797074954296161No Hit
GAGGACATCTCATGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCTG100.9140767824497258No Hit
GAGGACATCTTATGGTGCACTGAAGCAGTGGTATCAACGCAGAGTACATG90.8226691042047531Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAGCAAGGGT80.7312614259597806No Hit
AAGGTGGATGGTGATGCCGCTGGTCTACTTCGCAGTCAAGACCAAGTCTC80.7312614259597806No Hit
TGCCATCTTGCCACATCTTATATTTCGGTCCCTTACGCCCCAAGGGTATC70.6398537477148081No Hit
TGCCATCTTGCCACATCTTATATTTCGGTCCCTTACGCCCCGAGGGTATC70.6398537477148081No Hit
TGCCATCTTGCCACATCAGGGTATCCGCCAACCAGTCAAGACCAAGTCTC60.5484460694698354No Hit
TGCCATCTTGCCACATCTGGAAGGGTATCCGCCAACCAGTCAAGACCAAG60.5484460694698354No Hit
TGCCATCTTGCCACATCAAGGGTATCCGCCAACACAGAAGGGTATCCGCC50.4570383912248629No Hit
AAGGTGGATGGTGATGTGGTCAGTGTGCAAACTGAGGTAGCGACACCAGT50.4570383912248629No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACCAGTCAAGACCAAGTCTC50.4570383912248629No Hit
TGCCATCTTGCCACATCTTAGGGTATCCGCCAACCAGTCAAGACCAAGTC50.4570383912248629No Hit
TGCCATCTTGCCACATCTGGGTATCCGCCAACACAGAAGGGTATCCGCCA50.4570383912248629No Hit
GAGGACATCTTATGGTGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCT50.4570383912248629No Hit
GAGGACATCTTATGGGGGCAAACTGAGTACAGTCAAGACCAAGTCTCTGC40.3656307129798903No Hit
TGCCATCTTGCCACATCTTAGGGGTATCCGCCAACCAGTCAAGACCAAGT40.3656307129798903No Hit
TGCCATCTTGCCACATCTTGTATTTAAGTCGTCTGCAAAGGATTCGGCCC40.3656307129798903No Hit
TGCCATCTTGCCACATCTTGGTATCCGCCAACACAGAAGGGTATCCGCCA40.3656307129798903No Hit
TGCCATCTTGCCACATCTTGAAGGCAGGAAGGGTATCCGCCAACCAGTCA40.3656307129798903No Hit
TGCCATCTTGCCACATCTGGGTATCCGCCAACCAGTCAAGACCAAGTCTC40.3656307129798903No Hit
TGCCATCTTGCCACATCTGTACCAGCTGCTAATGCTGGCTCAACAAGGGT30.2742230347349177No Hit
TGCCATCTTGCCACATCTTAGGCCAAGCAGTGGTATCAACGCAGAGTACA30.2742230347349177Clontech Universal Primer Mix Long (96% over 29bp)
TGCCATCTTGCCACATCTAGGGTATCCGCCAACCAGTCAAGACCAAGTCT30.2742230347349177No Hit
TGCCATCTTGCCACATCTTAGTATCCGCCAACACAGAAGGGTATCCGCCA30.2742230347349177No Hit
TGCCATCTTGCCACATCTTGGGTATCCGCCAACCAGTCAAGACCAAGTCT30.2742230347349177No Hit
TGCCCTCTCTGCCTTTTCTCCCCCAGGGTATTTGGTTTCCCAGTCCACTA30.2742230347349177No Hit
GAGGACATCTTATGGGGGCAACTGAGGTACAGTCAAGACCAAGTCTCTGC30.2742230347349177No Hit
GAGGACATCTTATGGTGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCT30.2742230347349177No Hit
GAGGACATCTTATGGGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTCT30.2742230347349177No Hit
AAGGTGGATGGTGATGTGGTTAAGCAGTGGTATCAACGCAGAGTACATGG30.2742230347349177Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTTGGGGTATCCGCCAACCAGTCAAGACCAAGTC30.2742230347349177No Hit
GAGGACATCTTATGGTGGCAAATGAGGTACAGTCAAGACCAAGTCTCTGC20.18281535648994515No Hit
TGCCATCTTGCCACATCTGCCAAGGGTATCCGCCAACCAGTCAAGACCAA20.18281535648994515No Hit
TGCCATCTTGCCACATCTCCTCAAAGGGTATCCGCCAACCAGTCAAGACC20.18281535648994515No Hit
TGCCATCTTGCCACATCGGAAGGGTATCCGCCAACCAGTCAAGACCAAGT20.18281535648994515No Hit
AGGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTA20.18281535648994515No Hit
TGCCATCTTGCCACATCTTGAGGGTATCCGCCAACCAGTCAAGACCAAGT20.18281535648994515No Hit
AGGTGGATGGTGATGTGGTCAAGCAGTGGTATCAACGCAGAGTACATGGG20.18281535648994515Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGGACATCTTATGGGGGCAAACTGAGGTCAGTCAAGACCAAGTCTCTGC20.18281535648994515No Hit
TGCCATCTTGCCACATCTATCACGAAGGGTATCCGCCAACCAGTCAAGAC20.18281535648994515No Hit
TGCCATCTTGCCACATCTTCGCCCAAGCAGTGGTATCAACGCAGAGTACA20.18281535648994515Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCATCTTGCCACATCTCAAGCCAAGCAGTGGTATCAACGCAGAGTACA20.18281535648994515Clontech Universal Primer Mix Long (96% over 26bp)
TGCCATCTTGCCACATCTTGGAGGGTATCCGCCAACCAGTCAAGACCAAG20.18281535648994515No Hit
TGCCATCTTGCCACATCTTAGGTATCCGCCAACCAGTCAAGACCAAGTCT20.18281535648994515No Hit
AAGTGGATGGTGATGTGGTCTACTTCGCAGTCAAGACCAAGTCTCTGCTA20.18281535648994515No Hit
AAGGTGGATGGTGATGTGGTCAGTGTATGGATGGGTATCCGCCAACCAGT20.18281535648994515No Hit
GAGGACATCTTATGGGGGCAAACTAGGTACAGTCAAGACCAAGTCTCTGC20.18281535648994515No Hit
TGCCATCTTGCCACATCTTGAAGGCAGTAAGGGTATCCGCCAACCAGTCA20.18281535648994515No Hit
TGCCATCTTGCCACATCTTAGGTATCCGCCAACACAGAAGGGTATCCGCC20.18281535648994515No Hit
TGCCATCTTGCCACATCTGGAGGGTATCCGCCAACCAGTCAAGACCAAGT20.18281535648994515No Hit
GAGGACATCTTATGGTGGGGCAAACTGAGGTACAGTCAAGACCAAGTCTC20.18281535648994515No Hit
TGCCATCTTGCCACATCTGCCAACACAGGGTATCCGCCAACCAGTCAAGA20.18281535648994515No Hit
TGCCATCTTGCCACATCTTGGGGGGGTATCCGCCAACCAGTCAAGACCAA20.18281535648994515No Hit
TGCCATCTTGCCACATCTTAGTATCCGCCAACCAGTCAAGACCAAGTCTC20.18281535648994515No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)